What are different sterilization methods used in laboratory?
The most common methods of sterilization practised in lab are
- Dry heat: Specimen containing bacteria is exposed to high temperature
- Wet heat: Pressurised steam is used to kill microbes, for example, autoclave that is like pressure cooker that produces steam.
- Filtration: Filtration is used where filters are as small as 0.2um is used
- Radiation: UV has limited penetration, so it is generally safe to use although it is less effective to X-rays and gamma rays. X-rays and gamma rays are used only for special purposes only
- Solvent: Solvent like ethanol and iso-propanol kills microbial cells but not the spores